Recombinant Gelsolin (GSN)

Organism Species: Rattus norvegicus (Rat)

Art.No: BES2372Ra

specifications: 50ug

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Ala432~Ala780

Tags: N-terminal His Tag

Subcellular Location: Secreted

Purity: > 97%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% SKL, 5% Trehalose and Proclin300.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.3

Predicted Molecular Mass: 39.6kDa

Accurate Molecular Mass: 43kDa as determined by SDS-PAGE reducing conditions.

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do notvortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

        Store at 2-8ºC for one month.

      Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

[ SEQUENCE ]

AQHGMDDD GTGQKQIHRIEGSNKVLVDP ATYGOFYGGD SYIILYNYRH GGROGOIIYN WOGAOSTODEVAASAILTAOLDEELGGTPV OSRVVOGKEP AHLMSLFGGK PMIIYKGGTS

RDGGQTTPAS TRLFOVRASS SGATRAVEVM PKAGALNSND AFVLKTPSAA

YLWVGTGASD AEKTGALELL KVLRAQHVOV EEGSEPDGFW EALGGKTAYR

TSPRLKDKKM DAHPPRLFAC SNRIGRFVIE EVPGELMQED LATDDVMLLD

TWDOVFVWVG KDSOEEEKTE ALTSAKRYIETDPANRDRRT PITVVROGFE

PPSFVGWFLG WDDDYWSVDP LDRALAELAA

[ IDENTIFICATION ]

Figure. SDS-PAGE

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.