ACVR2B Protein, Human, Recombinant (ECD, hFc Tag): 产品信息

纯度:> 97 % as determined by SDS-PAGE

内毒素:< 1.0 EU per μg of the protein as determined by the LAL method

生物活性:1. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.02-0.1 µg/mL in the presence of 10 ng/mL recombinant Activin A.

2. Measured by its binding ability in a functional ELISA. Immobilized Inhibin Human, Mouse, Rat, Cynomolgus, Rhesus Inhibin beta A/Activin A (Cat:10429-HNAH) at 2 μg/ml (100 μl/well) can bind Human ACVR2B hFc(Cat:10229-H02H), the EC50 of Human ACVR2B hFc is 12-60 ng/mL.

蛋白构建:A DNA sequence encoding the N-terminal segment (Met 1-Thr 134) from the extracellular domain of human ACVR2B was expressed with the fused human IgG1 Fc region at the C-terminus.

表达宿主:HEK293 Cells

种属:Human

预测 N 端:Ser 19

分子量:The recombinant human ACVR2B/Fc chimera is a disulfide-linked homodimer generated after removal of the signal peptide. The reduced monomer comprises 354 amino acids and predicts a molecular mass of 40.0 kDa. As a result of glycosylation, the monomer migrates as an approximately 60-65 kDa protein in SDS-PAGE under reducing conditions.

缓冲液:Lyophilized from sterile PBS, pH 7.4

Please contact us for any concerns or special requirements.

Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hard copy of CoA.

运输方式:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件:Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

注意事项：该产品仅供科研实验使用，未经允许不得用于其它用途！