链霉亲和素偶联磁珠

Product Information

1. Product Description 

Streptavidin MagPoly beads are ideal for purification of biotinylated proteins and nucleic acids, here is primarily intended for isolation of human exosome subpopulations and flow cytometry analysis from cell culture media or urine. This product can be also used to prepare exosome subpopulations for western blot, electron microscopy and qRT-PCR etc.. Streptavidin MagPoly beads are uniform, superparamagnetic polystyrene beads (1.0μm diameter) coated with streptavidin. The beads need to be coated with your exosome specific antibody (biotinylated) prior to use. The antibody coupled beads are directly incubated with samples and captured exosomes are magnetically separated for downstream applications.

Table 1 Characteristics of Streptavidin MagPoly beads

For Research Use Only. Not for use in diagnostic procedures. 

2. Protocols The protocol uses 100μL Streptavidin MagPoly Beads, but this may be scaled up or down as required. 

2.1 Preparation of the Magnetic Beads 

1) Completely resuspend the beads by shaking or vortexing the vial. 

2) Transfer 100μL Streptavidin MagPoly Beads to a new tube. 

3) Place the tube on a magnetic separation rack to collect the beads at tube wall. Remove and discard the supernatant. 

4) Add 0.5 mL selected washing buffer ( 0.1 M phosphate, 0.15 M NaCl, pH 7.0 ) to the tube and invert the tube several times to mix. Use the magnetic separation rack to collect the beads and discard the supernatant. Repeat this step twice. 

2.2 Method for Couple magnetic beads with biotinylated antibody Biotinylated antibody: Use approximately 2-5mg of biotinylated antibody/mL settled Streptavidin MagPoly Beads. 

1) Resuspend the beads in 100μL Binding/Washing Buffer ( 0.1 M phosphate, 0.15 M NaCl, 0.1% BSA, pH 7.0 ). 

2) Add biotinylated antibody solution to the beads and gently invert tube to mix. 

3) Incubate the tube at room temperature with mixing (on a shaker or rotator) for one hour. 

4) Use the magnetic separation rack to collect the beads and discard the supernatant. 

5) Add 1.0 mL Binding/Washing Buffer to the tube and mix well, use the magnetic separation rack to collect the beads and discard the supernatant. Repeat the wash step three times. 

6) Resuspend the antibody bound beads in 100μL Binding/Washing Buffer. 

7) Add antigen sample to the tube and gently invert tube to mix. Incubate at room temperature for 30 minutes to overnight at 4°C. 

8) Wash the beads with 1mL Binding/Wash Buffer. Use the magnetic separation rack to collect the beads and discard the supernatant. Repeat the wash step three times. 

9) Add 100μL Binding/Washing Buffer to restore the magnetic bead.

Antibody-coupled magnetic beads can be stored at 2℃ to 8℃ for several months in Binding/Washing Buffer with 0.02 % sodium azide as a preservative (dependent on the stability of the primary antibody).

用途：该产品仅供科研实验使用，未经允许不得用于其它用途！以实际收货产品说明书为准，网站说明书仅供参考。