Recombinant Gelsolin (GSN)
Organism Species: Rattus norvegicus (Rat)
Art.No: BES2372Ra
specifications: 50ug
[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Ala432~Ala780
Tags: N-terminal His Tag
Subcellular Location: Secreted
Purity: > 97%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% SKL, 5% Trehalose and Proclin300.
Original Concentration: 200µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.3
Predicted Molecular Mass: 39.6kDa
Accurate Molecular Mass: 43kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do notvortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
[ SEQUENCE ]
AQHGMDDD GTGQKQIHRIEGSNKVLVDP ATYGOFYGGD SYIILYNYRH GGROGOIIYN WOGAOSTODEVAASAILTAOLDEELGGTPV OSRVVOGKEP AHLMSLFGGK PMIIYKGGTS
RDGGQTTPAS TRLFOVRASS SGATRAVEVM PKAGALNSND AFVLKTPSAA
YLWVGTGASD AEKTGALELL KVLRAQHVOV EEGSEPDGFW EALGGKTAYR
TSPRLKDKKM DAHPPRLFAC SNRIGRFVIE EVPGELMQED LATDDVMLLD
TWDOVFVWVG KDSOEEEKTE ALTSAKRYIETDPANRDRRT PITVVROGFE
PPSFVGWFLG WDDDYWSVDP LDRALAELAA
[ IDENTIFICATION ]
Figure. SDS-PAGE
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.