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Anti-Lamin A/C LMNA Antibody BES2069LCA
| 货号 | BES2069LCA |
|---|---|
| 产品名称 | Anti-Lamin A/C LMNA Antibody |
| 基因名 | LMNA |
| 抗体来源 | Chicken |
| 克隆 | Polyclonal |
| 抗体亚型 | Chicken |
| 分子量 | 74KD |
| 免疫原 | Full length recombinant human lamin A protein expressed in and purified from E. coli. |
| 内容 | Supplied as an aliquot of concentrated IgY preparation plus 5mM NaN3 |
| 浓度 | 500 ug/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Lamins are structural protein components of the nuclear lamina, a protein network underlying the inner nuclear membrane that determines nuclear shape and size. There are three types of lamins, A,B and C. The lamin A/C (LMNA) gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-lamin A and lamin C. Lamin A/C is mapped to 1q21.2-q21.3 and mutations in this gene cause a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Lamin A/C deficiency is thus associated with both defective nuclear mechanics and impaired mechanically activated gene transcription. |
| 研究类别 | 1. Lammerding, J.; Schulze, P. C.; Takahashi, T.; Kozlov, S.; Sullivan, T.; Kamm, R. D.; Stewart, C. L.; Lee, R. T. : Lamin A/C deficiency causes defective nuclear mechanics and mechanotransduction. J. Clin. Invest. 113: 370-378, 2004.2. Lin, F.; Worman, H. J. : Structural organization of the human gene encoding nuclear lamin A and nuclear lamin C. J. Biol. Chem. 268: 16321-16326, 1993.3. Wydner, K. L.; McNeil, J. A.; Lin, F.; Worman, H. J.; Lawrence, J. B. : Chromosomal assignment of human nuclear envelope protein genes LMNA, LMNB1, and LBR by fluorescence in situ hybridization. Genomics 32: 474-478, 1996. |
![Western blot analysis of cytosolic or nuclear enriched fractions of cell lines probed with chicken pAb to lamin A/C, M00438-2, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa cytosol, [3] HeLa nuclear, [4] NIH-3T3 cytosol, and [5] NIH-3T3 nuclear fractions. Two strong bands at 74 and 65kDa correspond to lamin A and lamin C proteins respectively, detected exclusively in the nuclear fractions. The same blot was simultaneously probed with mouse mAb to GAPDH, in red. The single band at 37kDa represents GAPDH protein which is expressed predominantly in the cytosolic fractions.](/upload/ueditor/image/20211124/619ddb6f64575.png "Western blot analysis of cytosolic or nuclear enriched fractions of cell lines probed with chicken pAb to lamin A/C, M00438-2, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa cytosol, [3] HeLa nuclear, [4] NIH-3T3 cytosol, and [5] NIH-3T3 nuclear fractions. Two strong bands at 74 and 65kDa correspond to lamin A and lamin C proteins respectively, detected exclusively in the nuclear fractions. The same blot was simultaneously probed with mouse mAb to GAPDH, in red. The single band at 37kDa represents GAPDH protein which is expressed predominantly in the cytosolic fractions.")
用鸡 pAb 探测到 lamin A/C，M00438-2，绿色稀释 1:1,000：[1] 蛋白质标准品（红色），[2] HeLa 细胞溶质，[3 ] HeLa 核，[4] NIH-3T3 细胞质，和 [5] NIH-3T3 核组分。74 和 65kDa 的两个强条带分别对应于核纤层蛋白 A 和核纤层蛋白 C 蛋白，仅在核组分中检测到。同样的印迹同时用小鼠 mAb 探测到 GAPDH，红色。37kDa 处的单条带代表 GAPDH 蛋白，其主要在细胞质部分中表达。