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Anti-Lamin A/C LMNA Monoclonal Antibody BES2070LCA
| 货号 | BES2070LCA |
|---|---|
| 产品名称 | Anti-Lamin A/C LMNA Monoclonal Antibody |
| 基因名 | LMNA |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal |
| 抗体亚型 | Mouse |
| 分子量 | 74KD |
| 免疫原 | Full length recombinant human lamin A expressed in and purified from E. coli. |
| 内容 | Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3 |
| 浓度 | 500 ug/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Lamins are structural protein components of the nuclear lamina, a protein network underlying the inner nuclear membrane that determines nuclear shape and size. There are three types of lamins, A,B and C. The lamin A/C (LMNA) gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-lamin A and lamin C. Lamin A/C is mapped to 1q21.2-q21.3 and mutations in this gene cause a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Lamin A/C deficiency is thus associated with both defective nuclear mechanics and impaired mechanically activated gene transcription. |
| 研究类别 | 1. Lammerding, J.; Schulze, P. C.; Takahashi, T.; Kozlov, S.; Sullivan, T.; Kamm, R. D.; Stewart, C. L.; Lee, R. T. : Lamin A/C deficiency causes defective nuclear mechanics and mechanotransduction. J. Clin. Invest. 113: 370-378, 2004.2. Lin, F.; Worman, H. J. : Structural organization of the human gene encoding nuclear lamin A and nuclear lamin C. J. Biol. Chem. 268: 16321-16326, 1993.3. Wydner, K. L.; McNeil, J. A.; Lin, F.; Worman, H. J.; Lawrence, J. B. : Chromosomal assignment of human nuclear envelope protein genes LMNA, LMNB1, and LBR by fluorescence in situ hybridization. Genomics 32: 474-478, 1996. |
![Western blot analysis of different cell lysates using mouse mAb to lamin A/C, M00438-1, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa, [3] HEK293 [4] C6, and [5] NIH-3T3 cell lysates. Two strong bands at 74 and 65kDa correspond to the lamin A and lamin C proteins respectively, detected only in the cells of human origin. M00438-1 antibody failed to recognize rat or mouse proteins.](/upload/ueditor/image/20211124/619ddbf4c3c73.png "Western blot analysis of different cell lysates using mouse mAb to lamin A/C, M00438-1, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa, [3] HEK293 [4] C6, and [5] NIH-3T3 cell lysates. Two strong bands at 74 and 65kDa correspond to the lamin A and lamin C proteins respectively, detected only in the cells of human origin. M00438-1 antibody failed to recognize rat or mouse proteins.")
使用小鼠 mAb 对 lamin A/C 的不同细胞裂解物进行蛋白质印迹分析，M00438-1，绿色稀释 1:1,000：[1] 蛋白质标准（红色），[2] HeLa，[3] HEK293 [4] C6， [5] NIH-3T3 细胞裂解物。74 和 65kDa 的两个强条带分别对应于核纤层蛋白 A 和核纤层蛋白 C 蛋白，仅在人源细胞中检测到。抗体无法识别大鼠或小鼠蛋白质。