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SARS-CoV-2 Spike S1 (P9L, C136F, 144-145 deletion, R190S, D215G, Y449H, E484K, N501Y, D614G, H655Y, N679K) Protein (His Tag) : 产品信息
纯度:> 95 % as determined by SDS-PAGE
内毒素:< 1.0 EU per μg protein as determined by the LAL method.
生物活性:Immobilized human ACE2 protein (mFc tag)(Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S1 (P9L, C136F, 144-145 deletion, R190S, D215G, Y449H, E484K, N501Y, D614G, H655Y, N679K) Protein (His Tag)(Cat:40591-V08H37), the EC50 is 12-80 ng/mL.
蛋白构建:A DNA sequence encoding the SARS-CoV-2 Spike S1 with mutations P9L, C136F, 144-145 deletion, R190S, D215G, Y449H, E484K, N501Y, D614G, H655Y, N679K) (Met1-Arg685) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant C.1.2) which emerged in the South Africa.
表达宿主:HEK293 Cells
种属:SARS-CoV-2
预测 N 端:Val 16
分子量:The recombinant SARS-CoV-2 Spike S1 consists of 679 amino acids and predicts a molecular mass of 76.06 kDa. As a result of glycosylation, it migrates as an approximately 114.84 kDa band in SDS-PAGE under reducing conditions.
缓冲液:Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件:Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.