SARS-CoV-2 B.1.617.2 Spike S1+S2 trimer Protein (ECD, His tag) : 产品信息
纯度:> 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
内毒素:< 1.0 EU per μg protein as determined by the LAL method.
生物活性:Immobilized human ACE2 protein (mFc tag)(Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 B.1.617.2 Spike S1+S2 trimer Protein (ECD, His tag)(Cat:40589-V08H10),the EC50 of SARS-CoV-2 B.1.617.2 Spike S1+S2 trimer Protein (ECD, His tag)(Cat:40589-V08H10) is 3-20 ng/mL.
蛋白构建:A DNA sequence encoding the SARS-CoV-2 Spike S1+S2 with mutations T19R, E156G, 157-158 deletion, L452R, T478K, D614G, F817P, A892P, A899P, A942P, D950N, K986P, V987P and furin cleavage site mutants) expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.617.2) which emerged in the India.
表达宿主:HEK293 Cells
种属:SARS-CoV-2
预测 N 端:Val 16
分子量:The recombinant SARS-CoV-2 Spike S1+S2 consists of 1229 amino acids and predicts a molecular mass of 136.21 kDa.
缓冲液:Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件:Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.