Recombinant Perforin 1 (PRF1)

Organism Species: Mus musculus (Mouse)

Instruction manua

规格：50μg

[ PROPERTIES ] 

Source: Prokaryotic expression

Host: E.coli

Residues: Val40~Lys355

Tags: N-terminal His and GST Tag

Subcellular Location: Membrane, Secreted, Cytoplasm, Endosome

Purity: > 90%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% Sarcosyl, 5%Trehalose.

Original Concentration: 400μg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 7.4

Predicted Molecular Mass: 65.3kDa

Accurate Molecular Mass: 65kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage:  Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

Figure.Gene Sequencing (extract)

[ IDENTIFICATION ]

Figure.Gene Sequencing (extract)

[ IMPORTANT NOTE ]  

The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.